Abstract
The anchorage-independence of cells is closely related to their tumorigenicity. In the screening of inhibitors of anchorage-independent growth of tumor cells, anicemycin was isolated from the fermentation broth of an actinomycete strain TP-A0648. The producing strain was isolated from a leaf of Aucuba japonica collected in Toyama, Japan and identified as Streptomyces sp. based on the taxonomic data. The structure of anicemycin was elucidated as a new analog of spicamycin by NMR and MS analysis. Anicemycin inhibited the anchorage-independent growth of the human ovary cancer SKOV-3 cells with an IC50 of 0.015 microM about three times more potently than their anchorage-dependent growth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antibiotics, Antineoplastic / biosynthesis
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Antibiotics, Antineoplastic / chemistry
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Antibiotics, Antineoplastic / pharmacology*
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Cell Proliferation / drug effects
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Chemical Phenomena
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Chemistry, Physical
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Drug Screening Assays, Antitumor
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Fermentation
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Humans
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Microscopy, Electron, Scanning
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Models, Molecular
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Plant Leaves / microbiology
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Purine Nucleosides / biosynthesis
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Purine Nucleosides / chemistry
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Purine Nucleosides / pharmacology*
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Spectrophotometry, Ultraviolet
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Streptomyces / classification
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Streptomyces / metabolism*
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Streptomyces / ultrastructure
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Structure-Activity Relationship
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Tumor Cells, Cultured
Substances
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Antibiotics, Antineoplastic
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Purine Nucleosides
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anicemycin