Allelic expression and quantitative RT-PCR study of TAp73 and DeltaNp73 in non-Hodgkin's lymphomas

Leuk Res. 2006 Feb;30(2):170-7. doi: 10.1016/j.leukres.2005.06.024. Epub 2005 Aug 3.

Abstract

p73 shares structural and functional homology to p53. p73 generates different proteins using alternative promoters and splicing which have different biological characteristics. We investigated the pattern (monoallelic or biallelic) of expression of TAp73 and DeltaNp73 in normal lymphocytes and lymphomas using two p73 polymorphisms. We found monoallelic expression of TAp73 in normal lymphocytes and tumors, and a selective expression of AT allele in all cases. Moreover, the quantitative expression analysis revealed DeltaNp73 over-expression in both B- and T-cell lymphomas comparing with normal lymphoid cells, suggesting a role in tumorigenesis. Finally, we have confirmed that although DeltaNp73 over-expression could be an alternative mechanism of p53 inactivation, both alterations may appear together.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Alleles
  • Child
  • DNA-Binding Proteins / analysis
  • DNA-Binding Proteins / genetics*
  • Female
  • Humans
  • Lymphoma, Non-Hodgkin / genetics*
  • Male
  • Middle Aged
  • Nuclear Proteins / analysis
  • Nuclear Proteins / genetics*
  • Protein Isoforms
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Suppressor Protein p53 / analysis
  • Tumor Suppressor Proteins / analysis
  • Tumor Suppressor Proteins / genetics*

Substances

  • DNA-Binding Proteins
  • Nuclear Proteins
  • Protein Isoforms
  • Tumor Suppressor Protein p53
  • Tumor Suppressor Proteins