Abstract
A bacterial primer set, known to produce a 542-bp amplicon specific for Bacteroides thetaiotaomicron, generated this product in PCR with 1 ng of extracted DNA from 92% of 25 human fecal samples, 100% of 20 sewage samples, and 16% of 31 dog fecal samples. The marker was not detected in 1 ng of fecal DNA from 61 cows, 35 horses, 44 pigs, 24 chickens, 29 turkeys, and 17 geese.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Animals
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Animals, Domestic / microbiology
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Bacteroides / genetics*
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Cattle
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DNA, Bacterial / analysis*
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DNA, Bacterial / isolation & purification
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Dogs
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Feces / microbiology*
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Genetic Markers / genetics*
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Humans
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Sensitivity and Specificity
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Sewage / microbiology
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Water Pollution / analysis*
Substances
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DNA, Bacterial
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Genetic Markers
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Sewage