U-2940, a human B-cell line derived from a diffuse large cell lymphoma sequential to Hodgkin lymphoma

Int J Cancer. 2006 Feb 1;118(3):555-63. doi: 10.1002/ijc.21417.

Abstract

Several patterns of association between Hodgkin and non-Hodgkin lymphomas are recognized, some of which support a common cellular origin or shared transformation events for both malignancies. We describe the U-2940 cell line derived from a diffuse large B-cell lymphoma with some features consistent with mediastinal large B-cell lymphoma, clinically apparent 1 month after the initial course of chemotherapy for Hodgkin's disease, fulfilling the criteria for composite malignancies. U-2940 cells display a mature B phenotype with hypermutated IgH rearrangement typical of germinal/postgerminal center origin. The cell line is negative for Epstein-Barr virus and no evidence of t(14;18) was found. U-2940 cells display multiple chromosomal rearrangements similar to recurrent aberrations described in both Hodgkin and non-Hodgkin lymphomas, also partially shared by U-2932 derived from a B-cell lymphoma sequential to Hodgkin's disease. The original large B-cell lymphoma and the U-2940 cell line bear microsatellite instability, an abnormality associated with particular subtypes of non-Hodgkin lymphomas and found in tissues involved by Hodgkin lymphoma. Therefore, U-2940 cells bear several features known to occur in Hodgkin and in non-Hodgkin lymphomas, leading to the assumption that this cell line may constitute a useful tool to address elective pathways of lymphomagenesis and eventually the Hodgkin and non-Hodgkin lymphoma association.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Animals
  • Cell Line, Tumor
  • Chromosome Aberrations
  • Chromosomes, Human, Pair 14 / genetics
  • Chromosomes, Human, Pair 18 / genetics
  • Colony-Forming Units Assay
  • DNA, Neoplasm / analysis
  • Epstein-Barr Virus Infections / etiology
  • Epstein-Barr Virus Infections / pathology
  • Female
  • Gene Rearrangement
  • Herpesvirus 4, Human / pathogenicity
  • Hodgkin Disease / drug therapy
  • Hodgkin Disease / pathology*
  • Humans
  • Immunoglobulin Heavy Chains / genetics
  • Lymphoma, B-Cell / genetics
  • Lymphoma, B-Cell / pathology*
  • Lymphoma, Large B-Cell, Diffuse / pathology*
  • Mediastinal Neoplasms / pathology*
  • Mice
  • Mice, Nude
  • Neoplasms, Second Primary / etiology
  • Neoplasms, Second Primary / pathology*
  • Spectral Karyotyping
  • Translocation, Genetic

Substances

  • DNA, Neoplasm
  • Immunoglobulin Heavy Chains