Abstract
Anti-HIV-1 gp120 single chain antibody(scFv) gene and staphylococcus extoxin A(SEA) gene were inserted into vector pPIC9K. The recombinant plasmid was integrated into Pichia pastoris by electroporation. High level expression was performed by determining the Muts phenotype and screening muti-copy integrants. The recombinant protein was about 57kD and the production was 50.1 mg/L. It was shown that the two kinds of protein affected the conformation of each other by antibody affinity assay, but the recombinant targeting toxins could highly mediate CTLs to kill HIV-1 target cells.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Enterotoxins / genetics
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Genetic Vectors
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HIV Envelope Protein gp120 / biosynthesis
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HIV Envelope Protein gp120 / genetics*
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HIV Envelope Protein gp120 / immunology
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HIV-1 / genetics
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HIV-1 / immunology*
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Immunoglobulin Variable Region / biosynthesis
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Immunoglobulin Variable Region / genetics*
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Immunoglobulin Variable Region / immunology
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Pichia / genetics
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Pichia / metabolism*
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
Substances
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Enterotoxins
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HIV Envelope Protein gp120
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Immunoglobulin Variable Region
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Recombinant Proteins
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enterotoxin A, Staphylococcal