Method to detect minimal amounts of calcium dissolved in acidic solutions

The study describes the application of the Arsenazo III method for detection of minimal amounts of calcium 12.4-49.4 micromol/l in different acidic solutions (hydrochloric acid, oxalic acid, maleic acid, phosphoric acid, tartaric acid, citric acid, lactic acid and acetic acid) adjusted to pH 2.0, 2.3 and 3.0. A mixture of the respective calcium concentrations with distilled water served as control. The experiments were run with ten repeats in series. Assessment of intra- and interassay coefficient of variation, and lower limit of quantification revealed that depending on the acid used, the Arsenazo III method is a reliable tool to quantify minimal calcium contents in acidic solutions.