Biofunctionalization of fluorescent-magnetic-bifunctional nanospheres and their applications

Chem Commun (Camb). 2005 Sep 14:(34):4276-8. doi: 10.1039/b508075d. Epub 2005 Aug 8.

Abstract

Hydrazide-containing bifunctional nanospheres were covalently coupled on the surface with IgG, avidin, and biotin, to generate novel fluorescent-magnetic-biotargeting trifunctional nanospheres, which can be used in a number of biomedical applications, including visual sorting and manipulation of apoptotic cells as demonstrated here.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldehydes / chemistry
  • Annexin A5
  • Apoptosis / radiation effects
  • Avidin / chemistry
  • Biotin / chemistry
  • Chromatography, Liquid
  • Fluorescein-5-isothiocyanate
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Humans
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G / chemistry
  • Magnetics
  • Nanoparticles / chemistry*
  • Ultraviolet Rays

Substances

  • Aldehydes
  • Annexin A5
  • Fluorescent Dyes
  • Immunoglobulin Fc Fragments
  • Immunoglobulin G
  • Avidin
  • Biotin
  • Fluorescein-5-isothiocyanate