Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2'-amino-alpha-l-LNA

Patrick J Hrdlicka; T Santhosh Kumar; Jesper Wengel

doi:10.1039/b506986f

Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2'-amino-alpha-l-LNA

Chem Commun (Camb). 2005 Sep 14:(34):4279-81. doi: 10.1039/b506986f. Epub 2005 Jul 21.

Authors

Patrick J Hrdlicka¹, T Santhosh Kumar, Jesper Wengel

Affiliation

¹ Nucleic Acid Center, Department of Chemistry, University of Southern Denmark, DK-5230 Odense M, Denmark.

PMID: 16113721
DOI: 10.1039/b506986f

Abstract

Incorporation of a single pyrene-functionalized 2'-amino-alpha-l-LNA monomer X into short DNA strands induces extraordinarily high binding affinity towards complementary DNA (up to 16 degrees C increase per modification), whereas labile duplexes, suitable as probes for targeting of double stranded DNA, are formed upon positioning of two monomers X in an interstrand +1 zipper motif.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Nucleic Acid Denaturation
Protein Denaturation / radiation effects
Pyrenes / chemistry*
RNA / chemistry
Spectrometry, Fluorescence
Thymidine / analogs & derivatives*
Thymidine / chemistry
Ultraviolet Rays

Substances

2'-N-(pyren-1-yl)methyl-2'-amino-LNA monomer
Pyrenes
RNA
DNA
Thymidine