Abstract
The EtOAc-soluble fraction from a methanolic extract of Hovenia dulcis Thunb. exhibited neuroprotective activity against glutamate-induced neurotoxicity in mouse hippocampal HT22 cells. The neuroprotective activity-guided isolation resulted in 8 phenolic compounds (1-8), such as vanillic acid (1), ferulic acid (2), 3,5-dihydroxystilbene (3), (+)-aromadendrin (4), methyl vanillate (5), (-)-catechin (6), 2,3,4-trihydrobenzoic acid (7), and (+)-afzelechin (8). Among these, compounds 6 and 8 had a neuroprotective effect on the glutamate-induced neurotoxicity in HT22 cells. Furthermore, compound 6 had a DPPH free radical scavenging effect with an IC50 value of 57.7 microM, and a superoxide anion radical scavenging effect with an IC50 value of 8.0 microM. Both compounds 6 and 8 had ABTS cation radical scavenging effects with IC50 values of 7.8 microM and 23.7 microM, respectively. These results suggest that compounds 6 and 8 could be neuroprotectants owing to their free radical scavenging activities.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzopyrans / isolation & purification
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Benzopyrans / pharmacology
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Benzothiazoles
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Biphenyl Compounds
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Catechin / isolation & purification
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Catechin / pharmacology*
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Cell Line
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Flavonoids
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology*
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Glutamic Acid
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Inhibitory Concentration 50
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Mice
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Neurons / drug effects
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Neuroprotective Agents / isolation & purification
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Neuroprotective Agents / pharmacology*
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Phenols / isolation & purification
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Phenols / pharmacology
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Picrates / metabolism
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Plant Bark
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Plant Extracts / chemistry
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Plant Extracts / pharmacology*
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Plants, Medicinal
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Rhamnaceae*
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Sulfonic Acids / metabolism
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Superoxides / metabolism
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Tannins / isolation & purification
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Tannins / pharmacology*
Substances
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Benzopyrans
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Benzothiazoles
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Biphenyl Compounds
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Flavonoids
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Free Radical Scavengers
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Neuroprotective Agents
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Phenols
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Picrates
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Plant Extracts
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Sulfonic Acids
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Tannins
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Superoxides
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2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
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Glutamic Acid
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Catechin
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1,1-diphenyl-2-picrylhydrazyl
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afzelechin