Abstract
Pixantrone is an immunesuppressor similar to mitoxantrone but with lower cardiotoxicity. We evaluated the effect of pixantrone on B cells and lymphomononuclear cells in the course of acute EAE. Pixantrone reduced the number of B cells and suppressed myelin basic protein (MBP) specific IgG production. In vitro, pixantrone induced apoptosis of rat B lymphocytes in a way similar to mitoxantrone. In addition, pixantrone inhibited antigen specific and mitogen induced lymphomononuclear cell proliferation, as well as IFN-gamma production, during EAE. These findings suggest a similar mechanism of action for pixantrone and mitoxantrone on the effector function of lymphomonocyte B and T cells.
MeSH terms
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Analysis of Variance
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Animals
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Antibodies / blood
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Antigens, CD / immunology
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Antigens, CD / metabolism
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Apoptosis / drug effects
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B-Lymphocytes / drug effects*
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B-Lymphocytes / physiology
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Cell Proliferation / drug effects
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Cytokines / metabolism
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Cytokinesis
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Disease Models, Animal
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Encephalomyelitis, Autoimmune, Experimental / drug therapy*
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Enzyme-Linked Immunosorbent Assay / methods
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Female
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Flow Cytometry / methods
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Immunosuppressive Agents / therapeutic use*
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Isoquinolines / therapeutic use*
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Myelin Basic Protein / administration & dosage
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Myelin Basic Protein / immunology
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Rats
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Rats, Inbred Lew
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T-Lymphocytes / drug effects
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Time Factors
Substances
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Antibodies
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Antigens, CD
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Cytokines
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Immunosuppressive Agents
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Isoquinolines
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Myelin Basic Protein
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pixantrone