Abstract
Haemophilus influenzae type b (Hib) was grown in continuous culture under cystine-limitation between dilution rates (D) of 0.065-0.28 h-1. A similar outer-membrane protein profile, as adjudged by SDS-PAGE, was found at all dilution rates. However, a shift to a lipopolysaccharide structure with a greater electrophoretic mobility on SDS-PAGE with accompanying changes in monoclonal antibody reactivity was observed at D greater than or equal to 0.15 h-1. Growth rate per se can affect the expression of outer-membrane components of Hib.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Outer Membrane Proteins / analysis*
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Bacterial Outer Membrane Proteins / biosynthesis
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Bacteriological Techniques
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Culture Media
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Cystine / metabolism
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Electrophoresis, Polyacrylamide Gel
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Haemophilus influenzae / chemistry
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Haemophilus influenzae / growth & development*
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Haemophilus influenzae / metabolism
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Immunoblotting
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Lipopolysaccharides / analysis*
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Lipopolysaccharides / biosynthesis
Substances
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Bacterial Outer Membrane Proteins
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Culture Media
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Lipopolysaccharides
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Cystine