Tissue microarray analysis of the prognostic value of E-cadherin, Ki67, p53, p27, survivin and MSH2 expression in upper urinary tract transitional cell carcinoma

Eur Urol. 2005 Nov;48(5):764-70. doi: 10.1016/j.eururo.2005.07.005. Epub 2005 Aug 1.

Abstract

Objectives: Invasive upper urinary tract transitional cell carcinoma (UUT-TCC) has a poor prognosis (survival <50% at 5 years), and tumor stage and grade often fail to predict outcome. Our purpose was to establish whether the expression of Ki67, p53, p27, E-cadherin, survivin or MSH2 can provide prognostic information in UUT-TCC.

Methods: The following data from the files of 62 patients treated for UUT-TCC over 12 years were collated: age at diagnosis, prior history of cancer, tobacco consumption, tumor stage (including surgical margins) and grade, and disease progression. Immunohistochemistry (IHC) for Ki67, p53, p27, E-cadherin, survivin and MSH2 was performed on tissue microarray sections from tumor tissue.

Results: Overall, 31 patients died with metastasis from UUT-TCC. Mean survival was 20+/-16 months (range 2-83). In a univariate analysis, advanced age (>68 years), high stage, and loss of E-cadherin and high Ki67 expression were associated with a poor prognosis and disease recurrence. In a multivariate analysis, the independent factors of prognosis and recurrence were E-cadherin (p=0.001; p=0.004), age (p=0.022; p=0.008), and high stage (p=0.023; p=0.008).

Conclusions: E-cadherin is a useful independent prognostic factor in UUT-TCC, for use in addition to age and tumor stage. It is of particular interest to predict recurrence in patients with low grade non-invasive tumors. Ki67 expression is informative but less significant. Survivin, p53, p27 and MSH2 have no prognostic value.

MeSH terms

  • Aged
  • Biomarkers, Tumor / metabolism
  • Cadherins / metabolism*
  • Carcinoma, Transitional Cell / diagnosis
  • Carcinoma, Transitional Cell / metabolism*
  • Carcinoma, Transitional Cell / pathology
  • Disease Progression
  • Female
  • Humans
  • Inhibitor of Apoptosis Proteins
  • Ki-67 Antigen / metabolism*
  • Male
  • Microarray Analysis
  • Microtubule-Associated Proteins / metabolism*
  • Middle Aged
  • Multivariate Analysis
  • MutS Homolog 2 Protein / metabolism*
  • Neoplasm Proteins / metabolism*
  • Prognosis
  • Proliferating Cell Nuclear Antigen / metabolism*
  • Survival Rate
  • Survivin
  • Tumor Suppressor Protein p53 / metabolism*
  • Urologic Neoplasms / diagnosis
  • Urologic Neoplasms / metabolism*
  • Urologic Neoplasms / pathology

Substances

  • BIRC5 protein, human
  • Biomarkers, Tumor
  • Cadherins
  • Inhibitor of Apoptosis Proteins
  • Ki-67 Antigen
  • Microtubule-Associated Proteins
  • Neoplasm Proteins
  • Proliferating Cell Nuclear Antigen
  • Survivin
  • Tumor Suppressor Protein p53
  • p27 antigen
  • MSH2 protein, human
  • MutS Homolog 2 Protein