Subunit interaction determines IKs participation in cardiac repolarization and repolarization reserve

Circulation. 2005 Sep 6;112(10):1384-91. doi: 10.1161/CIRCULATIONAHA.105.543306. Epub 2005 Aug 29.

Abstract

Background: The role of IKs, the slow delayed rectifier K+ current, in cardiac ventricular repolarization has been a subject of debate.

Methods and results: We develop a detailed Markov model of IKs and its alpha-subunit KCNQ1 and examine their kinetic properties during the cardiac ventricular action potential at different rates. We observe that interaction between KCNQ1 and KCNE1 (the beta-subunit) confers kinetic properties on IKs that make it suitable for participation in action potential repolarization and its adaptation to rate changes; in particular, the channel develops an available reserve of closed states near the open state that can open rapidly on demand.

Conclusions: Because of its ability to form an available reserve, IKs can function as a repolarization reserve when IKr, the rapid delayed rectifier, is reduced by disease or drug and can prevent excessive action potential prolongation and development of arrhythmogenic early afterdepolarizations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Action Potentials / physiology*
  • Animals
  • Anura
  • Computer Simulation
  • Guinea Pigs
  • Humans
  • Ion Channel Gating / physiology*
  • KCNQ1 Potassium Channel / chemistry
  • KCNQ1 Potassium Channel / physiology*
  • Kinetics
  • Long QT Syndrome / physiopathology
  • Markov Chains
  • Models, Biological*
  • Myocardial Contraction / physiology*
  • Oocytes / physiology
  • Potassium Channels, Voltage-Gated / physiology
  • Protein Subunits / physiology
  • Reproducibility of Results

Substances

  • KCNE1 protein, human
  • KCNQ1 Potassium Channel
  • Potassium Channels, Voltage-Gated
  • Protein Subunits