mRNA translation is not a prerequisite for small interfering RNA-mediated mRNA cleavage

Differentiation. 2005 Jul;73(6):287-93. doi: 10.1111/j.1432-0436.2005.00029.x.

Abstract

RNA interference constitutes a major means of eliminating mRNAs, yet how the small interfering RNAs (siRNA) within the RNA-induced silencing complex (RISC) finds its homologous target in the cell remains unknown. An attractive hypothesis is that RNA interference is linked to translation which allows RISC ready access to every translated mRNA. To test whether translation could direct siRNAs to mRNAs, chemical and biological inhibitors of translation and their effects on mRNA cleavage were tested. Our results show that mRNA degradation by siRNAs is not dependent on mRNA translation.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / biosynthesis
  • Actins / genetics
  • Cells, Cultured
  • Clotrimazole / metabolism
  • Humans
  • Iron / metabolism
  • Protein Biosynthesis / drug effects
  • Protein Biosynthesis / genetics*
  • RNA Interference*
  • RNA, Messenger / metabolism*
  • RNA, Small Interfering / metabolism*

Substances

  • Actins
  • RNA, Messenger
  • RNA, Small Interfering
  • Iron
  • Clotrimazole