Five new triterpene glycosides from Lysimachia foenum-graecum and evaluation of their effect on the arachidonic acid metabolizing enzyme

Planta Med. 2005 Aug;71(8):770-5. doi: 10.1055/s-2005-871289.

Abstract

Five new oleanane-type triterpene saponins, named foenumosides A ( 1), B ( 2), C ( 3), D ( 4) and E ( 5), were isolated from the aerial parts of Lysimachia foenum-graecum Hance. Their structures were identified on the basis of 1D and 2D NMR techniques, including H-H COSY, HMQC, HMBC, HMQC-TOCSY, ROESY experiments as well as chemical methods. We have evaluated the cytotoxity of 1 - 5 against rat and human polymorphonuclear leukocytes and the effect of 5 on the arachidonic acid metabolizing enzyme. All compounds showed a high degree of toxicity except for compound 5, while 5 notably reduced the production of leukotriene B (4) (LTB (4)) from rat peritoneal leukocytes with an IC (50) value of 74 microM without inhibiting human elastase. Compound 5 also reduced the production of 12-HHTrE and 12-HETE by 14 % and 50 % as a measurement for cyclooxygenase-1 and 12-lipoxygenase inhibition at 100 microM.

MeSH terms

  • Animals
  • Arachidonic Acid / metabolism*
  • Glycosides / administration & dosage
  • Glycosides / chemistry
  • Glycosides / pharmacology
  • Glycosides / therapeutic use
  • Humans
  • Inhibitory Concentration 50
  • Leukocytes / drug effects
  • Leukocytes / enzymology
  • Phytotherapy*
  • Plant Components, Aerial
  • Plant Extracts / administration & dosage
  • Plant Extracts / chemistry
  • Plant Extracts / pharmacology*
  • Plant Extracts / therapeutic use
  • Primulaceae*
  • Rats
  • Triterpenes / administration & dosage
  • Triterpenes / chemistry
  • Triterpenes / pharmacology
  • Triterpenes / therapeutic use

Substances

  • Glycosides
  • Plant Extracts
  • Triterpenes
  • Arachidonic Acid