Cloning of short hairpin RNAs for gene knockdown in mammalian cells
Nat Methods
.
2004 Nov;1(2):163-7.
doi: 10.1038/nmeth1104-163.
Authors
Patrick J Paddison
1
,
Michele Cleary
,
Jose Maria Silva
,
Kenneth Chang
,
Nihar Sheth
,
Ravi Sachidanandam
,
Gregory J Hannon
Affiliation
1
Cold Spring Harbor Laboratory, 1 Bungtown Road, Cold Spring Harbor, New York 11724, USA.
PMID:
16144086
DOI:
10.1038/nmeth1104-163
No abstract available
MeSH terms
Animals
Cloning, Molecular / methods*
DNA Primers / metabolism
Mammals
Polymerase Chain Reaction / methods
RNA Interference*
RNA, Small Interfering / metabolism*
Substances
DNA Primers
RNA, Small Interfering