PCR-based diagnosis of neonatal staphylococcal bacteremias

J Clin Microbiol. 2005 Sep;43(9):4823-5. doi: 10.1128/JCM.43.9.4823-4825.2005.

Abstract

We compared PCR with blood cultures in the diagnosis of neonatal staphylococcal sepsis. Significant association was observed between PCR-based and culture-based diagnosis of staphylococcal bacteremia. Positive and negative predictive values for PCR were 100% and 98%, respectively. These data indicate that PCR may serve as a useful adjunct for the rapid diagnosis of staphylococcal sepsis.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacteremia / diagnosis*
  • Bacteremia / microbiology
  • Bacteriological Techniques
  • Blood / microbiology
  • Culture Media
  • DNA, Bacterial / analysis
  • Female
  • Humans
  • Infant
  • Infant, Low Birth Weight
  • Infant, Newborn
  • Infant, Premature
  • Infant, Premature, Diseases / diagnosis*
  • Infant, Premature, Diseases / microbiology
  • Intensive Care Units, Neonatal
  • Male
  • Polymerase Chain Reaction / methods*
  • Predictive Value of Tests
  • Sensitivity and Specificity
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Staphylococcus / classification
  • Staphylococcus / genetics
  • Staphylococcus / isolation & purification*

Substances

  • Culture Media
  • DNA, Bacterial