Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

A T Hesselink; A J C van den Brule; Z M A Groothuismink; M Molano; J Berkhof; C J L M Meijer; P J F Snijders

doi:10.1128/JCM.43.9.4868-4871.2005

Comparison of three different PCR methods for quantifying human papillomavirus type 16 DNA in cervical scrape specimens

J Clin Microbiol. 2005 Sep;43(9):4868-71. doi: 10.1128/JCM.43.9.4868-4871.2005.

Authors

A T Hesselink¹, A J C van den Brule, Z M A Groothuismink, M Molano, J Berkhof, C J L M Meijer, P J F Snijders

Affiliation

¹ Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands.

Abstract

We compared real-time LightCycler and TaqMan assays and the GP5+/6+ PCR/enzyme immunoassay (EIA) to assess the human papillomavirus type 16 (HPV16) load in cervical scrape specimens. Both real-time PCR assays determined the HPV16 load in scrape specimens similarly. The level of agreement between these assays and the GP5+/6+ PCR/EIA was low (P = 0.004), suggesting that the latter method is not suited for quantifying HPV16 DNA.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Cervix Uteri / virology*
DNA Probes, HPV
DNA, Viral / analysis*
Female
Humans
Immunoenzyme Techniques
Papillomaviridae / genetics
Papillomaviridae / isolation & purification*
Papillomavirus Infections / diagnosis*
Papillomavirus Infections / virology
Polymerase Chain Reaction / methods*
Taq Polymerase
Vaginal Smears

Substances

DNA Probes, HPV
DNA, Viral
Taq Polymerase