Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay

Rachaneeporn Tiyawisutsri; Sharon J Peacock; Sayan Langa; Direk Limmathurotsakul; Allen C Cheng; Wirongrong Chierakul; Wipada Chaowagul; Nicholas P J Day; Vanaporn Wuthiekanun

doi:10.1128/JCM.43.9.4872-4874.2005

Antibodies from patients with melioidosis recognize Burkholderia mallei but not Burkholderia thailandensis antigens in the indirect hemagglutination assay

J Clin Microbiol. 2005 Sep;43(9):4872-4. doi: 10.1128/JCM.43.9.4872-4874.2005.

Authors

Rachaneeporn Tiyawisutsri¹, Sharon J Peacock, Sayan Langa, Direk Limmathurotsakul, Allen C Cheng, Wirongrong Chierakul, Wipada Chaowagul, Nicholas P J Day, Vanaporn Wuthiekanun

Affiliation

¹ Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand.

Abstract

The indirect hemagglutination assay routinely used to detect antibodies to Burkholderia pseudomallei was modified to detect cross-reactivity of antibodies to B. pseudomallei, B. mallei, and B. thailandensis antigens. We demonstrate a lack of cross-reactivity between B. pseudomallei and B. thailandensis but marked cross-reactivity between B. pseudomallei and B. mallei.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Antibodies, Bacterial / blood
Antibodies, Bacterial / immunology*
Antigens, Bacterial / immunology*
Burkholderia / immunology*
Burkholderia mallei / immunology*
Cross Reactions
Female
Hemagglutination Tests
Humans
Male
Melioidosis / immunology*
Melioidosis / microbiology
Middle Aged

Substances

Antibodies, Bacterial
Antigens, Bacterial