[Isolation culture and identification of human neural stem cells from human embryos]

Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2004 Apr;29(2):129-31.
[Article in Chinese]

Abstract

Objective: To obtain and culture the human neural stem cells from the aborted human embryos.

Methods: The neural stem cells were isolated and purified by the specific proliferous culture system of neural stem cells, and the molecular maker and multi-potency of the obtained neural stem cells were identified.

Results: Human neural stem cells, which were isolated from 8 - 10 month old aborted human embryos, could express nestin ( a kind of specified antigen of the neural stem cell), and it could be differentiate into neurons and glials.

Conclusion: Neural stem cells can exist in human embryos, and it can be expanded in vitro.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Differentiation / drug effects
  • Cell Separation
  • Cells, Cultured
  • Embryo, Mammalian / cytology*
  • Humans
  • Neurons / cytology*
  • Stem Cells / cytology*