Abstract
The mammalian Per1 gene is one of the most important components of circadian clock function of the suprachiasmatic nucleus and peripheral tissues. We examined whether the beta2-adrenoceptor agonists, procaterol and fenoterol, induce human Per1 mRNA expression in human bronchial epithelium. The in vitro stimulation of beta2-adrenoceptor agonists in BEAS-2B cells led to a remarkable increase in the level of hPer1 mRNA. Moreover, fenoterol or procaterol induced the phosphorylation of CREB in BEAS-2B cells as verified by immunoblot analysis. beta2-adrenoceptor agonists induced human Per1 mRNA expression by the signaling pathways of cAMP-CREB in BEAS-2B cells.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adrenergic beta-2 Receptor Agonists*
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Adrenergic beta-Agonists / pharmacology*
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Bronchi / drug effects*
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Bronchi / metabolism
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Cell Cycle Proteins
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Cell Line
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Epithelial Cells / drug effects
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Epithelial Cells / metabolism
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Fenoterol / pharmacology*
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Gene Expression / drug effects
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Humans
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Nuclear Proteins / biosynthesis*
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Period Circadian Proteins
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Procaterol / pharmacology*
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RNA, Messenger / metabolism
Substances
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Adrenergic beta-2 Receptor Agonists
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Adrenergic beta-Agonists
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Cell Cycle Proteins
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Nuclear Proteins
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PER1 protein, human
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Period Circadian Proteins
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RNA, Messenger
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Fenoterol
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Procaterol