Abstract
A technique was developed to obtain viable cytoplasmic droplets, enabling the formation of a gigaohm seal. Such cytoplasmic droplets can be used for characterization of plasma membrane ion channels from filamentous fungi by patch-clamp technique. Two K(+) ionic channels are characterized with a conductance of 43 pS and 74 pS.
MeSH terms
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Cell Membrane Permeability / physiology*
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Cytoplasm / chemistry
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Cytoplasm / ultrastructure
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Electrophysiology / instrumentation
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Electrophysiology / methods*
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Fungi / growth & development*
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Fungi / ultrastructure
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Ion Channels / physiology*
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Membrane Potentials / physiology
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Models, Biological
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Patch-Clamp Techniques
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Phycomyces / physiology*
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Potassium Channels / physiology
Substances
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Ion Channels
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Potassium Channels