Abstract
Centrosomes act as sites of microtubule growth, but little is known about how the number and stability of microtubules emanating from a centrosome are controlled during the cell cycle. We studied the role of the TACC3-XMAP215 complex in this process by using purified proteins and Xenopus laevis egg extracts. We show that TACC3 forms a one-to-one complex with and enhances the microtubule-stabilizing activity of XMAP215 in vitro. TACC3 enhances the number of microtubules emanating from mitotic centrosomes, and its targeting to centrosomes is regulated by Aurora A-dependent phosphorylation. We propose that Aurora A regulation of TACC3 activity defines a centrosome-specific mechanism for regulation of microtubule polymerization in mitosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Aurora Kinases
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Cell Cycle Proteins / physiology*
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Cell Extracts
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Centrosome / physiology*
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Kinesins / metabolism
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Microtubule-Associated Proteins / metabolism
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Microtubule-Associated Proteins / physiology
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Microtubules / chemistry
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Microtubules / metabolism*
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Mitosis*
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Oocytes / chemistry
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Phosphorylation
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Protein Kinases / physiology*
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Protein Serine-Threonine Kinases
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Transcription Factors / physiology*
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Xenopus Proteins / metabolism
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Xenopus Proteins / physiology*
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Xenopus laevis
Substances
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CKAP5 protein, Xenopus
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Cell Cycle Proteins
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Cell Extracts
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KIF2C protein, human
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Microtubule-Associated Proteins
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TACC3 protein, Xenopus
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Transcription Factors
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Xenopus Proteins
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Protein Kinases
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AURKA protein, Xenopus
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Aurora Kinases
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Protein Serine-Threonine Kinases
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Kinesins