Abstract
We have synthesized the first steroid hormone-MR contrast agent conjugate designed to track the cell signaling process upon binding to a gene switch system. The derivative has a high relaxivity and when tested in vitro is active as a progesterone antagonist (RU-486). By combining a transcriptional system and a noninvasive imaging technology, such as MRI, it would be a powerful tool to research the cell signaling pathway in vivo.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Binding, Competitive / drug effects
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Gadolinium / chemistry*
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Ligands
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Magnetic Resonance Imaging / methods*
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Mifepristone / chemical synthesis*
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Mifepristone / chemistry
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Molecular Conformation
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Organometallic Compounds / chemical synthesis*
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Organometallic Compounds / chemistry
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Protein Binding
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Receptors, Progesterone / drug effects
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Signal Transduction / drug effects
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Signal Transduction / physiology*
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Structure-Activity Relationship
Substances
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Ligands
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Organometallic Compounds
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Receptors, Progesterone
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Mifepristone
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Gadolinium