FASL -844C polymorphism is associated with increased activation-induced T cell death and risk of cervical cancer

J Exp Med. 2005 Oct 3;202(7):967-74. doi: 10.1084/jem.20050707. Epub 2005 Sep 26.

Abstract

The FAS receptor-ligand system plays a key role in regulating apoptotic cell death, and corruption of this signaling pathway has been shown to participate in tumor-immune escape and carcinogenesis. We have recently demonstrated (Sun, T., X. Miao, X. Zhang, W. Tan, P. Xiong, and D. Lin. 2004. J. Natl. Cancer Inst. 96:1030-1036; Zhang, X., X. Miao, T. Sun, W. Tan, S. Qu, P. Xiong, Y. Zhou, and D. Lin. 2005. J. Med. Genet. 42:479-484) that functional polymorphisms in FAS and FAS ligand (FASL) are associated with susceptibility to lung cancer and esophageal cancer; however, the mechanisms underlying this association have not been elucidated. We show that the FAS -1377G, FAS -670A, and FASL -844T variants are expressed more highly on ex vivo-stimulated T cells than the FAS -1377A, FAS -670G, and FASL -844C variants. Moreover, activation-induced cell death (AICD) of T cells carrying the FASL -844C allele was increased. We also found a threefold increased risk of cervical cancer among subjects with the FASL -844CC genotype compared with those with the -844TT genotype in a case-control study in Chinese women. Together, these observations suggest that genetic polymorphisms in the FAS-FASL pathway confer host susceptibility to cervical cancers, which might be caused by immune escape of tumor cells because of enhanced AICD of tumor-specific T cells.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antineoplastic Combined Chemotherapy Protocols
  • Case-Control Studies
  • Cell Death / genetics
  • Cell Death / immunology
  • China
  • Cyclophosphamide
  • Fas Ligand Protein
  • Female
  • Flow Cytometry
  • Genetic Predisposition to Disease*
  • Genotype
  • Haplotypes / genetics
  • HeLa Cells
  • Humans
  • Interleukin-2 / metabolism
  • Leukocytes, Mononuclear / metabolism
  • Linkage Disequilibrium
  • Melphalan
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / metabolism
  • Middle Aged
  • Polymorphism, Genetic*
  • Risk Factors
  • Semustine
  • T-Lymphocytes / metabolism*
  • Tumor Necrosis Factors / genetics*
  • Tumor Necrosis Factors / metabolism
  • Uterine Cervical Neoplasms / genetics*
  • Uterine Cervical Neoplasms / immunology

Substances

  • FASLG protein, human
  • Fas Ligand Protein
  • Interleukin-2
  • Membrane Glycoproteins
  • Tumor Necrosis Factors
  • Semustine
  • Cyclophosphamide
  • Melphalan

Supplementary concepts

  • MMC protocol