Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit

Roos S R Choudhurry; Damian C Melles; Kimberly Eadie; Margreet Vos; Heiman F L Wertheim; Henry A Verbrugh; Alex van Belkum; Willem B van Leeuwen

doi:10.1016/j.mimet.2005.08.009

Direct detection of human Staphylococcus aureus carriage in the nose using the Lightcycler Staphylococcus kit

J Microbiol Methods. 2006 May;65(2):354-6. doi: 10.1016/j.mimet.2005.08.009. Epub 2005 Sep 27.

Authors

Roos S R Choudhurry¹, Damian C Melles, Kimberly Eadie, Margreet Vos, Heiman F L Wertheim, Henry A Verbrugh, Alex van Belkum, Willem B van Leeuwen

Affiliation

¹ Erasmus MC, Department of Medical Microbiology and Infectious Diseases, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands.

PMID: 16194577
DOI: 10.1016/j.mimet.2005.08.009

Abstract

The Lightcycler Staphylococcus kit is a diagnostic tool for direct real-time detection of Staphylococcus aureus in clinical materials. We show here that detection of S. aureus nasal carriage using this test is hampered by competition of DNA from coagulase-negative staphylococci. However the test is well suited for species identification after culture and the identification of high-load S. aureus carriers.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Carrier State / microbiology*
Humans
Nasal Cavity / microbiology*
Polymerase Chain Reaction / methods*
Reagent Kits, Diagnostic*
Sensitivity and Specificity
Staphylococcal Infections / diagnosis*
Staphylococcal Infections / microbiology
Staphylococcus aureus / genetics
Staphylococcus aureus / isolation & purification*

Substances

Reagent Kits, Diagnostic