Abstract
Whipple disease (WD) is a rare systemic disease caused by Tropheryma whipplei and is characterized by the presence of foamy macrophages with periodic acid-Schiff-positive inclusions in tissues such as lamina propria. For the first time, we report the gene-expression profile of macrophages in intestinal lesions from a patient with WD. Microarray and real-time polymerase chain reaction revealed that genes encoding CCL18, cathepsins, scavenger receptor, interleukin-10, and lipid metabolites were up-regulated in intestinal lesions. This transcriptional pattern corresponds to that of M2/alternatively activated macrophages. Our results suggest that the T helper 2 response in the intestinal environment may account for the pathophysiological properties of WD.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinobacteria
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Aged
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Biopsy
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Cathepsins / genetics
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Cathepsins / metabolism
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Cells, Cultured
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Chemokines, CC / genetics
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Chemokines, CC / metabolism
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Duodenum / metabolism*
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Duodenum / physiopathology
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Foam Cells / metabolism
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Gene Expression Profiling*
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Humans
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Interleukin-10 / genetics
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Interleukin-10 / metabolism
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Lipid Metabolism / genetics
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Macrophage Activation*
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Macrophages / metabolism*
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Macrophages / microbiology
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Male
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Mucous Membrane / metabolism
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Mucous Membrane / physiopathology
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Oligonucleotide Array Sequence Analysis
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Receptors, Scavenger / genetics
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Receptors, Scavenger / metabolism
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Up-Regulation
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Whipple Disease / metabolism*
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Whipple Disease / pathology
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Whipple Disease / physiopathology
Substances
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CCL18 protein, human
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Chemokines, CC
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Receptors, Scavenger
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Interleukin-10
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Cathepsins