Galactomannan and computed tomography-based preemptive antifungal therapy in neutropenic patients at high risk for invasive fungal infection: a prospective feasibility study

Clin Infect Dis. 2005 Nov 1;41(9):1242-50. doi: 10.1086/496927. Epub 2005 Sep 29.

Abstract

Background: Empirical antifungal therapy is the standard treatment for persistent or relapsing antibiotic-resistant neutropenic fever. However, overtreatment resulting in increased toxicity and treatment-related cost is a major shortcoming of such therapy. We assessed the feasibility of a "preemptive" approach based on the incorporation of sensitive, noninvasive diagnostic tests for consecutive high-risk neutropenic patients who had received fluconazole prophylaxis while avoiding empirical therapy.

Methods: A total of 136 treatment episodes for persons who were at risk of acquiring invasive fungal infection (IFI) were screened for the presence of galactomannan with an enzyme immunoassay. A diagnostic evaluation, which included thoracic computed tomography scanning (HRCT) and bronchoscopy with lavage, was performed on the basis of well-defined clinical, radiological, and microbiological criteria. Only seropositive patients and patients with a positive microbiological test result plus supportive radiological findings received liposomal amphotericin B.

Results: Neutropenic fever developed in 117 episodes, of which at least 41 episodes (35%) satisfied existing criteria for empirical antifungal therapy. However, our protocol-driven preemptive approach reduced the rate of antifungal use for these episodes from 35% to 7.7% (a 78% reduction) and led to the early initiation of antifungal therapy in 10 episodes (7.3%) that were clinically not suspected of being IFI. No undetected cases of invasive aspergillosis were identified; 1 case of zygomycosis was missed. Breakthrough candidemia was diagnosed by conventional culture techniques and was treated successfully. With use of a preemptive approach, the 12-week survival rate for patients with IFI was 63.6% (it was 63.1% for those with invasive aspergillosis).

Conclusion: Preemptive therapy based on enzyme immunoassay and HRCT reduced the exposure to expensive and potentially toxic drugs and offered effective antifungal control, but it failed to detect non-Aspergillus IFI.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Algorithms
  • Amphotericin B / therapeutic use*
  • Antifungal Agents / therapeutic use*
  • Aspergillosis / blood
  • Aspergillosis / complications
  • Aspergillosis / diagnosis
  • Aspergillosis / prevention & control
  • Feasibility Studies
  • Female
  • Galactose / analogs & derivatives
  • Humans
  • Lung Diseases, Fungal / blood
  • Lung Diseases, Fungal / complications
  • Lung Diseases, Fungal / diagnosis
  • Lung Diseases, Fungal / prevention & control
  • Male
  • Mannans / blood*
  • Middle Aged
  • Mycoses / blood
  • Mycoses / complications
  • Mycoses / diagnosis*
  • Mycoses / prevention & control*
  • Neutropenia / complications*
  • Prospective Studies
  • Risk Factors
  • Tomography, X-Ray Computed*

Substances

  • Antifungal Agents
  • Mannans
  • galactomannan
  • Amphotericin B
  • Galactose