Abstract
The E-Cadherin-catenin complex plays a critical role in epithelial cell-cell adhesion, polarization, and morphogenesis. Here, we have analyzed the mechanism of Drosophila E-Cadherin (DE-Cad) localization. Loss of function of the Drosophila exocyst components sec5, sec6, and sec15 in epithelial cells results in DE-Cad accumulation in an enlarged Rab11 recycling endosomal compartment and inhibits DE-Cad delivery to the membrane. Furthermore, Rab11 and Armadillo interact with the exocyst components Sec15 and Sec10, respectively. Our results support a model whereby the exocyst regulates DE-Cadherin trafficking, from recycling endosomes to sites on the epithelial cell membrane where Armadillo is located.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Armadillo Domain Proteins
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Cadherins / metabolism*
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Carrier Proteins / metabolism
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Cell Membrane / metabolism*
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Drosophila / metabolism
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Drosophila / ultrastructure
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Endosomes / metabolism*
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Epithelial Cells / metabolism
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Exocytosis / physiology
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Membrane Transport Proteins
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Models, Biological
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Oocytes / metabolism*
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Oocytes / ultrastructure
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Protein Transport / physiology
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Trans-Activators / metabolism
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Transcription Factors
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Vesicular Transport Proteins / genetics
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Vesicular Transport Proteins / metabolism*
Substances
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ARM protein, Drosophila
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Armadillo Domain Proteins
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Cadherins
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Carrier Proteins
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Drosophila Proteins
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Membrane Proteins
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Membrane Transport Proteins
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Sec15 protein, Drosophila
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Sec5 protein, Drosophila
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Sec6 protein, Drosophila
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Trans-Activators
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Transcription Factors
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Vesicular Transport Proteins
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sec10 protein, Drosophila