Abstract
Expression of the high-oxygen-affinity cytochrome cbb3 and cytochrome bd ubiquinol oxidases of Brucella suis was studied in vitro and in the intramacrophagic niche, which was previously proposed to be oxygen limited. The cytochrome cbb3 oxidase was exclusively expressed in vitro, whereas the cytochrome bd oxidase was preferentially used inside macrophages and contributed to intracellular bacterial replication.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aerobiosis
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Brucella suis / enzymology*
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Brucella suis / genetics
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Brucella suis / growth & development*
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Cell Line
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Cell Proliferation
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Electron Transport Complex IV / genetics
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Electron Transport Complex IV / metabolism
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Gene Expression Regulation, Bacterial
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Humans
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Macrophages / metabolism
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Macrophages / microbiology*
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Oxidoreductases / genetics
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Oxidoreductases / metabolism*
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Oxygen / metabolism*
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Transcription, Genetic
Substances
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Bacterial Proteins
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Oxidoreductases
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cbb3 oxidase
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Electron Transport Complex IV
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Oxygen