A novel mucosal vaccine against foot-and-mouth disease virus induces protection in mice and swine

Houhui Song; Zhiliang Wang; Dongxia Zheng; Weihuan Fang; Yong Li; Yueyong Liu; Zhendong Niu; Bingsheng Qiu

doi:10.1007/s10529-005-2727-4

A novel mucosal vaccine against foot-and-mouth disease virus induces protection in mice and swine

Biotechnol Lett. 2005 Nov;27(21):1669-74. doi: 10.1007/s10529-005-2727-4.

Authors

Houhui Song¹, Zhiliang Wang, Dongxia Zheng, Weihuan Fang, Yong Li, Yueyong Liu, Zhendong Niu, Bingsheng Qiu

Affiliation

¹ Centre for Molecular Microbiology, Institute of Microbiology, Chinese Academy of Sciences, 13# Zhongguancun Beiyitiao, 100080, Beijing, PR China. [email protected]

PMID: 16247672
DOI: 10.1007/s10529-005-2727-4

Abstract

Epitopes of a foot-and-mouth disease virus (FMDV) capsid protein VP1 complex and a chimera of 6xHis-tagged cholera toxin B subunit (hCTB) were expressed in Hansenula polymorpha and used together as a mucosal vaccine. Antibody and cytokine responses to VP1-hCTB vaccine and protection against FMDV were evaluated by ELISA and a virus challenge test in mice, respectively. VP1-hCTB directly enhanced the expression of interleukin-5 (IL-5) both in serum and supernatants of cultured spleen cells. After challenging suckling mice with 10(5) FMDV (=50% lethal dosage per mouse) a greater protection was seen after intraperitoneal and intranasal vaccinations than after oral vaccination. In swine immunized with VP1-hCTB, immune responses were achieved after three administrations, and the vaccine protected swine (80%) when challenged with 10(6.5) FMDV (=50% infectious dosage per swine). These results demonstrated the possibility of using CTB as a mucosal adjuvant to elicit protective immune responses against FMDV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adjuvants, Immunologic / administration & dosage
Adjuvants, Immunologic / genetics
Animals
Antibodies, Viral / immunology*
Capsid Proteins / administration & dosage
Capsid Proteins / genetics
Capsid Proteins / immunology
Cells, Cultured
Cholera Toxin / administration & dosage
Cholera Toxin / genetics
Cholera Toxin / immunology
Female
Foot-and-Mouth Disease / immunology*
Foot-and-Mouth Disease / prevention & control
Foot-and-Mouth Disease Virus / genetics
Foot-and-Mouth Disease Virus / immunology*
Immunity, Mucosal*
Interleukin-5 / immunology
Mice
Mice, Inbred BALB C
Recombinant Fusion Proteins / administration & dosage
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / immunology
Spleen / cytology
Spleen / immunology
Swine
Viral Vaccines / administration & dosage
Viral Vaccines / immunology*

Substances

Adjuvants, Immunologic
Antibodies, Viral
Capsid Proteins
Interleukin-5
Recombinant Fusion Proteins
VP1 protein, Foot-and-mouth disease virus
Viral Vaccines
Cholera Toxin