Analysis of allele fidelity, polymorphic information content, and density of microsatellites in a genome-wide screening for hip dysplasia in a crossbreed pedigree

J Hered. 2005;96(7):847-53. doi: 10.1093/jhered/esi109. Epub 2005 Oct 26.

Abstract

Recent advances in genomics resources and tools are facilitating quantitative trait locus mapping. We developed a crossbreed pedigree for mapping quantitative trait loci for hip dysplasia in dogs by crossing dysplastic Labrador Retrievers and normal Greyhounds. We show that one advantage to using a crossbreed pedigree is the increased marker informativeness in the backcross/F2 population relative to the founder populations. We also discuss three factors that affect the detection power in the context of this crossbreed pedigree: being able to detect and correct genotyping errors, increasing marker density for chromosomes with a sparse coverage, and adding individuals to the mapping population as soon as they become available.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles*
  • Animals
  • Chromosome Mapping / methods
  • Dogs
  • Female
  • Hip Dysplasia, Canine / genetics*
  • Hybridization, Genetic
  • Male
  • Microsatellite Repeats / genetics*
  • Pedigree
  • Polymorphism, Genetic / genetics*
  • Reproducibility of Results
  • Research Design
  • Statistics as Topic / methods