Abstract
This chapter describes methods used to investigate implantation in the common marmoset monkey, Callithrix jacchus. A reverse-transcriptase polymerase chain reaction-strategy with which to detect transcripts for steroid receptors and enzymes involved in estradiol biosynthesis is described, and an immunohistochemistry approach for detecting proteins within the implantation site is presented.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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17-Hydroxysteroid Dehydrogenases / metabolism
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Animals
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Aromatase / metabolism
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Callithrix / physiology*
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Embryo Implantation / physiology*
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Estradiol / biosynthesis
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Estrogen Receptor alpha / genetics
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Estrogen Receptor alpha / metabolism
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Female
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Immunohistochemistry
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Models, Animal*
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Pregnancy / physiology*
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Progesterone / metabolism
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RNA / genetics
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RNA / metabolism
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Reproductive Techniques*
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Uterus / cytology
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Uterus / metabolism
Substances
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Estrogen Receptor alpha
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Progesterone
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Estradiol
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RNA
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17-Hydroxysteroid Dehydrogenases
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3 (or 17)-beta-hydroxysteroid dehydrogenase
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Aromatase