Intracellular interference of infectious bursal disease virus

J Virol. 2005 Nov;79(22):14437-41. doi: 10.1128/JVI.79.22.14437-14441.2005.

Abstract

A search for dominant-negative mutant polypeptides hampering infectious bursal disease virus (IBDV) replication has been undertaken. We have found that expression of a mutant version of the VP3 structural polypeptide known as VP3/M3, partially lacking the domain responsible for the interaction with the virus-encoded RNA polymerase, efficiently interferes with the IBDV replication cycle. Transformed cells stably expressing VP3/M3 show a significant reduction (up to 96%) in their ability to support IBDV growth. Our findings provide a new tool for the characterization of the IBDV replication cycle and might facilitate the generation of genetically modified chicken lines with a reduced susceptibility to IBDV infection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Birnaviridae Infections / physiopathology
  • Cell Line
  • Gene Deletion
  • Humans
  • Infectious bursal disease virus / genetics
  • Infectious bursal disease virus / physiology*
  • Mutation
  • Viral Structural Proteins / genetics
  • Viral Structural Proteins / physiology*
  • Virus Replication

Substances

  • VP3 protein, infectious bursal disease virus
  • Viral Structural Proteins