Abstract
Strategies and procedures for enriching, isolating, and cultivating reductively dechlorinating bacteria that use chloroorganic compounds as metabolic electron acceptors from environmental samples are described. Further, nucleic acid-based approaches used to detect and quantify dechlorinator (i.e., Dehalococcoides)-specific genes are presented.
Publication types
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Acetates / metabolism
-
Bacteria, Anaerobic / genetics
-
Bacteria, Anaerobic / growth & development
-
Bacteria, Anaerobic / isolation & purification*
-
Bacteria, Anaerobic / metabolism
-
Culture Media
-
DNA, Bacterial / isolation & purification
-
Drug Resistance, Bacterial
-
Hydrocarbons, Chlorinated / metabolism*
-
Hydrogen / metabolism
-
Hydrolases / genetics
-
Oxidation-Reduction
-
Polymerase Chain Reaction
-
RNA, Ribosomal, 16S / genetics
-
Soil Pollutants / metabolism
Substances
-
Acetates
-
Culture Media
-
DNA, Bacterial
-
Hydrocarbons, Chlorinated
-
RNA, Ribosomal, 16S
-
Soil Pollutants
-
Hydrogen
-
Hydrolases
-
2-haloacid dehalogenase