Abstract
Caveolin-1 has been implicated in apical transport of glycosylphosphatidylinositol (GPI)-anchored proteins and influenza virus hemagglutinin (HA). Here we have studied the role of caveolin-1 in apical membrane transport by generating caveolin-1-deficient Madin-Darby canine kidney (MDCK) cells using retrovirus-mediated RNA interference. The caveolin-1 knockdown (cav1-KD) MDCK cells were devoid of caveolae. In addition, caveolin-2 was retained in the Golgi apparatus in cav1-KD MDCK cells. However, we found no significant alterations in the apical transport kinetics of GPI-anchored proteins or HA upon depletion of caveolin-1. Similar results were obtained using embryonic fibroblasts from caveolin-1-knockout mice. Thus, we conclude that caveolin-1 does not play a major role in lipid raft-mediated biosynthetic membrane trafficking.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / metabolism
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Animals
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Biological Transport
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Biotin / chemistry
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Biotinylation
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Caveolin 1 / genetics
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Caveolin 1 / metabolism
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Caveolin 1 / physiology*
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Caveolin 2 / genetics
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Caveolin 2 / metabolism
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Cell Line
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Cell Membrane / metabolism
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Cholesterol / metabolism
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Dogs
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Dose-Response Relationship, Drug
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Fibroblasts / metabolism
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Glycosylphosphatidylinositols / metabolism
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Golgi Apparatus / metabolism
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Hemagglutinin Glycoproteins, Influenza Virus / metabolism
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Immunohistochemistry
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Kinetics
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Membrane Microdomains
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Mice
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Mice, Knockout
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Microscopy, Electron
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Microscopy, Fluorescence
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RNA Interference
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Retroviridae / genetics
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Retroviridae / metabolism
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Temperature
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Time Factors
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Transgenes
Substances
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Caveolin 1
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Caveolin 2
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Glycosylphosphatidylinositols
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Hemagglutinin Glycoproteins, Influenza Virus
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Biotin
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Cholesterol