Choroid plexus epithelial cells co-express the long and short form of the leptin receptor

Neurosci Lett. 2006 Jan 30;393(2-3):269-72. doi: 10.1016/j.neulet.2005.10.003. Epub 2005 Nov 2.

Abstract

Two types of leptin receptors (Ob-R) are structurally and functionally characterized. The Ob-Ra, also called short form of leptin receptor, and the Ob-Rb, which is functionally coupled to intracellular signalling pathways. In the CNS, the Ob-Ra is mainly present in the choroid plexus and in the blood-brain barrier and it is involved in leptin transport from the periphery to the brain. The Ob-Rb is widely distributed in the hypothalamus and in many peripheral tissues and is coupled to the JAK/STAT signalling pathway. In this work, we describe the presence of both Ob-Ra and Ob-Rb in a cell line of choroid plexus epithelium. In addition, we demonstrate that Ob-Rb in the choroid plexus is functionally coupled to intracellular signalling pathways as leptin induced the phosphorylation of the signal transducer and activator of transduction 3 (STAT3).

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Blotting, Western / methods
  • Cell Line, Tumor
  • Choroid Plexus / cytology*
  • Dose-Response Relationship, Drug
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism*
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / physiology*
  • Leptin / pharmacology
  • Receptors, Cell Surface / metabolism*
  • Receptors, Leptin
  • STAT3 Transcription Factor / metabolism
  • Sheep
  • Time Factors

Substances

  • Leptin
  • Receptors, Cell Surface
  • Receptors, Leptin
  • STAT3 Transcription Factor