Abstract
Recently we identified a bacterial factor (BimA) required for actin-based motility of Burkholderia pseudomallei. Here we report that Burkholderia mallei and Burkholderia thailandensis are capable of actin-based motility in J774.2 cells and that BimA homologs of these bacteria can restore the actin-based motility defect of a B. pseudomallei bimA mutant. While the BimA homologs differ in their amino-terminal sequence, they interact directly with actin in vitro and vary in their ability to bind Arp3.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actin-Related Protein 3 / metabolism
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Amino Acid Sequence
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Animals
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Bacterial Proteins / genetics
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Bacterial Proteins / physiology
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Burkholderia / genetics*
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Burkholderia / physiology*
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Burkholderia mallei / genetics
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Burkholderia mallei / physiology
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Burkholderia pseudomallei / genetics
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Burkholderia pseudomallei / physiology
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Cell Line
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Gene Deletion
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Genetic Complementation Test*
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Immunoblotting
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Macrophages / microbiology
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Mice
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Microfilament Proteins / genetics*
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Microfilament Proteins / physiology
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Microscopy, Confocal
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Molecular Sequence Data
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Mutation
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Protein Binding
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Sequence Homology, Amino Acid
Substances
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Actin-Related Protein 3
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Bacterial Proteins
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BimA protein, Burkholderia pseudomallei
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Microfilament Proteins