Abstract
The S-layer-encoding genes of 21 Lactobacillus helveticus strains were characterized. Phylogenetic analysis based on the identified S-layer genes revealed two main clusters, one which includes a sequence similar to that of the slpH1 gene of L. helveticus CNRZ 892 and a second cluster which includes genes similar to that of prtY. These results were further confirmed by Southern blot hybridization. This study demonstrates S-layer gene variability in the species L. helveticus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics*
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Base Sequence
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Blotting, Southern
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Genetic Variation*
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Lactobacillus helveticus / classification*
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Lactobacillus helveticus / genetics*
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics*
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Multigene Family
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Phylogeny
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Sequence Alignment
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Sequence Analysis, DNA
Substances
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Bacterial Proteins
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Membrane Glycoproteins
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S-layer proteins