Abstract
A TaqMan allelic-discrimination assay designed around a synonymous single-nucleotide polymorphism was used to genotype Burkholderia pseudomallei and Burkholderia mallei isolates. The assay rapidly identifies and discriminates between these two highly pathogenic bacteria and does not cross-react with genetic near neighbors, such as Burkholderia thailandensis and Burkholderia cepacia.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Base Sequence
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Burkholderia mallei / classification*
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Burkholderia mallei / genetics
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Burkholderia pseudomallei / classification*
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Burkholderia pseudomallei / genetics
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DNA Primers
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Genes, Bacterial / genetics
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Geography
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Molecular Sequence Data
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Nucleotides / genetics
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Polymerase Chain Reaction / methods*
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Polymorphism, Genetic
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Sequence Alignment
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Species Specificity
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Taq Polymerase
Substances
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DNA Primers
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Nucleotides
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Taq Polymerase