Abstract
UBP43 is the only deconjugating enzyme for the protein ISGylation system thus far identified. UBP43 activity is not critical for precursor processing, as UBP43-deficent cells can generate ISG15 conjugates upon type I interferon treatment. However, UBP43 deficiency caused a defect in the negative regulation of type I interferon signaling, resulting in enhanced and prolonged activation of Jak/Stat upon type I interferon treatment. This chapter describes the expression, purification, and enzymatic assays for UBP43.
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, P.H.S.
MeSH terms
-
Animals
-
Cloning, Molecular
-
Cytokines / genetics
-
Cytokines / metabolism*
-
Endopeptidases / biosynthesis*
-
Endopeptidases / genetics
-
Endopeptidases / isolation & purification*
-
Endopeptidases / metabolism
-
Humans
-
Mice
-
Recombinant Fusion Proteins / genetics
-
Recombinant Fusion Proteins / metabolism
-
Ubiquitin Thiolesterase
-
Ubiquitins / genetics
-
Ubiquitins / metabolism
-
beta-Galactosidase / genetics
-
beta-Galactosidase / metabolism
Substances
-
Cytokines
-
Recombinant Fusion Proteins
-
Ubiquitins
-
ISG15 protein, human
-
beta-Galactosidase
-
Endopeptidases
-
Usp18 protein, mouse
-
USP18 protein, human
-
Ubiquitin Thiolesterase