Abstract
We have produced human angiotensinogen in Chinese hamster ovary (CHO) cells. The expression products were purified to homogeneity by a single column chromatography and its 17 amino-terminal sequences were identical to those of the native protein. We demonstrated the recombinant human angiotensinogen to be a substrate for human renin.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Amino Acid Sequence
-
Angiotensinogen / biosynthesis
-
Angiotensinogen / genetics*
-
Angiotensinogen / isolation & purification
-
Animals
-
Blotting, Northern
-
CHO Cells
-
Chromatography, Liquid
-
Cloning, Molecular
-
Cricetinae
-
Electrophoresis, Polyacrylamide Gel
-
Humans
-
Molecular Sequence Data
-
RNA, Messenger / metabolism
-
Recombinant Proteins / biosynthesis
-
Recombinant Proteins / genetics
-
Recombinant Proteins / isolation & purification
-
Renin / metabolism
-
Substrate Specificity
Substances
-
RNA, Messenger
-
Recombinant Proteins
-
Angiotensinogen
-
Renin