Abstract
Human basic fibroblast growth factor has been expressed in Escherichia coli cells at a level of 2-3 mg/l culture, using a rapid procedure which requires only simple DNA manipulative work. The recombinant material has the same potency as natural basic fibroblast growth factor from bovine pituitary glands.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Base Sequence
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Blotting, Western
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Chromatography, High Pressure Liquid
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Escherichia coli / genetics*
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Fibroblast Growth Factor 2 / biosynthesis*
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Fibroblast Growth Factor 2 / chemistry
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Fibroblast Growth Factor 2 / genetics
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Humans
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Molecular Sequence Data
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Plasmids / genetics
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Transformation, Bacterial / genetics
Substances
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Recombinant Proteins
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Fibroblast Growth Factor 2