Abstract
Construction, genetic stability and protective efficacy of a recombinant fowlpox virus expressing hemagglutinin (HA) gene (rFPV-HA) of H5 subtype avian influenza virus were conducted. The results indicated that the recombinant fowlpox virus was obtained and purified by blue plaque selection. HA expressed by rFPV-HA was identified by indirect immunofluorescence array. The rFPV-HA could express reporter gene LacZ and HA gene after fifteen successive passages in chicken embryo fibroblast. The rFPV-HA induced completely protection of chickens against lethal challenge with homologous avian influenza virus. The results show that rFPV-HA has good genetic stability and antigenicity and should be a useful tool in HPAI control program by preventing illness and death in chickens when the disease spreads widely.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Viral / blood
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Chick Embryo
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Chickens
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Fowlpox virus / genetics*
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Fowlpox virus / metabolism
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Gene Expression
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Genetic Engineering*
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Hemagglutinin Glycoproteins, Influenza Virus / genetics
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Hemagglutinin Glycoproteins, Influenza Virus / immunology*
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Influenza A Virus, H5N1 Subtype / genetics
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Influenza A Virus, H5N1 Subtype / immunology*
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Influenza Vaccines / administration & dosage
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Influenza Vaccines / genetics
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Influenza Vaccines / immunology
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Influenza in Birds / immunology*
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Influenza in Birds / prevention & control
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Influenza in Birds / virology
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Poultry Diseases / immunology*
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Poultry Diseases / prevention & control
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Poultry Diseases / virology
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Random Allocation
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
Substances
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Antibodies, Viral
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Hemagglutinin Glycoproteins, Influenza Virus
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Influenza Vaccines
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Recombinant Proteins