Optical readout of gold nanoparticle-based DNA microarrays without silver enhancement

Biophys J. 2006 Jan 1;90(1):L13-5. doi: 10.1529/biophysj.105.076182. Epub 2005 Nov 11.

Abstract

We present a novel readout scheme for gold nanoparticle-based DNA microarrays relying on "Laser-Induced Scattering around a NanoAbsorber". It provides direct counting of individual nanoparticles present on each array spot and stable signals, without any silver enhancement. Given the detection of nanometer-sized particles, which minimize the steric hindrance, the linear dynamic range of the method is particularly large and well suited for microarray detection.

Publication types

  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acoustics
  • DNA / chemistry
  • Gold / chemistry*
  • Lasers
  • Microscopy, Fluorescence
  • Nanostructures / chemistry*
  • Nucleic Acid Hybridization
  • Oligonucleotide Array Sequence Analysis / instrumentation*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Optics and Photonics
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Silver / chemistry*

Substances

  • Silver
  • Gold
  • DNA