Abstract
Adducin regulates tubular absorption of sodium by modulating the expression levels of the sodium-potassium-ATPase in renal tubular cells. Adducin is a candidate gene in the pathogenesis of hypertension. Yeast two hybrid screen showed a specific interaction between human alpha Adducin and the regulatory factor for X box (RFX-1), a nuclear protein that down regulates the expression of several proteins in non neuronal cells. The interaction was confirmed in cells through co-immunoprecipitation and colocalization experiments. The binding of alpha Adducin to RFX-I and their nuclear co-localization suggests that Adducin can have a role in modulating the transcriptional regulating activity of RFX-I.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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COS Cells
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Calmodulin-Binding Proteins / analysis
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Calmodulin-Binding Proteins / metabolism*
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Cell Nucleus / chemistry
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Cell Nucleus / metabolism*
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Chlorocebus aethiops
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Library
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Humans
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Immunoprecipitation
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Kidney / metabolism*
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Regulatory Factor X Transcription Factors
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Regulatory Factor X1
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Transcription Factors / analysis
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Two-Hybrid System Techniques
Substances
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Calmodulin-Binding Proteins
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DNA-Binding Proteins
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RFX1 protein, human
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Regulatory Factor X Transcription Factors
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Regulatory Factor X1
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Transcription Factors
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adducin