Abstract
The merozoite surface protein 1 (MSP-1) is expressed in all Plasmodium species and is considered a major malaria vaccine candidate. We found that MSP-1 from Plasmodium vivax (PvMSP-1) contains a region of significant sequence homology with the 190L subunit vaccine derived from the P. falciparum MSP-1. The fragment, termed Pv200L, was expressed as a recombinant protein in Escherichia coli (rPv200L) and used to asses its immunologic relevance as a vaccine target. A cross-sectional, seroepidemiologic study conducted in Buenaventura, Colombia showed that 52.2% (95% confidence interval [CI] = 39.8-64.3) of individuals previously exposed to P. vivax and 72.8% (95% CI = 61.8-82.1) of P. vivax-infected patients had IgG antibodies to rPv200L. Immunization of BALB/c mice and Aotus monkeys induced IgG antibodies (titer > 10(6)) that cross-reacted with P. vivax parasites. Immunized monkeys displayed partial protection against a challenge with P. vivax blood stages. Our results suggest that Pv200L is a new malaria vaccine subunit and deserves further testing.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Antibodies, Protozoan / blood*
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Antigens, Protozoan / chemistry
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Antigens, Protozoan / genetics
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Antigens, Protozoan / immunology*
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Antigens, Protozoan / metabolism
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Antigens, Surface / chemistry
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Antigens, Surface / genetics
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Antigens, Surface / immunology*
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Antigens, Surface / metabolism
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Cebidae
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Colombia / epidemiology
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Cross-Sectional Studies
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Humans
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Immunization
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Immunoglobulin G / blood
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Malaria Vaccines / administration & dosage
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Malaria Vaccines / genetics
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Malaria Vaccines / immunology*
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Malaria, Vivax / epidemiology*
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Malaria, Vivax / immunology
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Malaria, Vivax / prevention & control*
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Merozoite Surface Protein 1 / chemistry*
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Mice
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Mice, Inbred BALB C
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Molecular Sequence Data
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Plasmodium vivax / immunology
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Plasmodium vivax / pathogenicity
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Recombinant Proteins / genetics
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Recombinant Proteins / immunology
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Recombinant Proteins / metabolism
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Seroepidemiologic Studies
Substances
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Antibodies, Protozoan
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Antigens, Protozoan
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Antigens, Surface
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Immunoglobulin G
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Malaria Vaccines
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Merozoite Surface Protein 1
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Recombinant Proteins
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Pv200 antigen, P vivax