Toward the clinical application of time-domain fluorescence lifetime imaging

I Munro; J McGinty; N Galletly; J Requejo-Isidro; P M P Lanigan; D S Elson; C Dunsby; M A A Neil; M J Lever; G W H Stamp; P M W French

doi:10.1117/1.2102807

Toward the clinical application of time-domain fluorescence lifetime imaging

J Biomed Opt. 2005 Sep-Oct;10(5):051403. doi: 10.1117/1.2102807.

Authors

I Munro¹, J McGinty, N Galletly, J Requejo-Isidro, P M P Lanigan, D S Elson, C Dunsby, M A A Neil, M J Lever, G W H Stamp, P M W French

Affiliation

¹ Imperial College London, Physics Department, Photonics Group, South Kensington Campus, London SW7 2AZ. [email protected]

PMID: 16292940
DOI: 10.1117/1.2102807

Abstract

High-speed (video-rate) fluorescence lifetime imaging (FLIM) through a flexible endoscope is reported based on gated optical image intensifier technology. The optimization and potential application of FLIM to tissue autofluorescence for clinical applications are discussed.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Computer Systems
Endoscopes*
Equipment Design
Equipment Failure Analysis
Fiber Optic Technology / instrumentation*
Image Enhancement / instrumentation*
Image Enhancement / methods
Image Interpretation, Computer-Assisted / instrumentation
Image Interpretation, Computer-Assisted / methods
Mice
Microscopy, Fluorescence / instrumentation*
Microscopy, Fluorescence / methods
Microscopy, Video / instrumentation*
Microscopy, Video / methods
Reproducibility of Results
Sensitivity and Specificity

Grants and funding

Wellcome Trust/United Kingdom