Effects of hypothalamic neurodegeneration on energy balance

PLoS Biol. 2005 Dec;3(12):e415. doi: 10.1371/journal.pbio.0030415. Epub 2005 Nov 29.

Abstract

Normal aging in humans and rodents is accompanied by a progressive increase in adiposity. To investigate the role of hypothalamic neuronal circuits in this process, we used a Cre-lox strategy to create mice with specific and progressive degeneration of hypothalamic neurons that express agouti-related protein (Agrp) or proopiomelanocortin (Pomc), neuropeptides that promote positive or negative energy balance, respectively, through their opposing effects on melanocortin receptor signaling. In previous studies, Pomc mutant mice became obese, but Agrp mutant mice were surprisingly normal, suggesting potential compensation by neuronal circuits or genetic redundancy. Here we find that Pomc-ablation mice develop obesity similar to that described for Pomc knockout mice, but also exhibit defects in compensatory hyperphagia similar to what occurs during normal aging. Agrp-ablation female mice exhibit reduced adiposity with normal compensatory hyperphagia, while animals ablated for both Pomc and Agrp neurons exhibit an additive interaction phenotype. These findings provide new insight into the roles of hypothalamic neurons in energy balance regulation, and provide a model for understanding defects in human energy balance associated with neurodegeneration and aging.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Agouti-Related Protein
  • Animals
  • Body Weight / genetics
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Eating
  • Energy Metabolism*
  • Gene Deletion
  • Genes, Reporter / genetics
  • High Mobility Group Proteins / genetics
  • High Mobility Group Proteins / metabolism
  • Hypothalamus / metabolism
  • Hypothalamus / pathology*
  • Hypothalamus / physiopathology*
  • Intercellular Signaling Peptides and Proteins / genetics
  • Intercellular Signaling Peptides and Proteins / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mutation / genetics
  • Nerve Degeneration / genetics
  • Nerve Degeneration / metabolism
  • Nerve Degeneration / physiopathology*
  • Pro-Opiomelanocortin / deficiency
  • Pro-Opiomelanocortin / genetics
  • Pro-Opiomelanocortin / metabolism
  • RNA, Messenger / genetics

Substances

  • AGRP protein, human
  • Agouti-Related Protein
  • Agrp protein, mouse
  • DNA-Binding Proteins
  • High Mobility Group Proteins
  • Intercellular Signaling Peptides and Proteins
  • RNA, Messenger
  • Tfam protein, mouse
  • Pro-Opiomelanocortin