Abstract
Neutralization of tumor necrosis factor alpha (TNF-alpha) significantly down-regulated antigen-induced lymphoproliferation and the expression of interleukin-12 p40 and gamma interferon mRNA and enhanced the viability of intracellular attenuated and virulent mycobacteria in cocultures of immune T cells and macrophages obtained from Mycobacterium bovis BCG-vaccinated guinea pigs. This suggests the crucial role of TNF-alpha in the activation of a type 1 T-cell response against Mycobacterium tuberculosis infection.
Publication types
-
Research Support, N.I.H., Extramural
MeSH terms
-
Animals
-
Antibodies / pharmacology
-
Antigens, Bacterial / immunology
-
Cell Proliferation
-
Coculture Techniques
-
Cytokines / metabolism*
-
Guinea Pigs
-
Interferon-gamma / genetics
-
Interferon-gamma / metabolism
-
Interleukin-12 / genetics
-
Interleukin-12 / metabolism
-
Interleukin-12 Subunit p40
-
Macrophages, Peritoneal / immunology
-
Macrophages, Peritoneal / microbiology*
-
Mycobacterium bovis / immunology
-
Mycobacterium tuberculosis / immunology*
-
Protein Subunits / genetics
-
Protein Subunits / metabolism
-
RNA, Messenger / analysis
-
RNA, Messenger / metabolism
-
Spleen / cytology
-
Spleen / immunology
-
Th1 Cells / immunology*
-
Tuberculosis / immunology
-
Tumor Necrosis Factor-alpha / antagonists & inhibitors*
-
Tumor Necrosis Factor-alpha / immunology
Substances
-
Antibodies
-
Antigens, Bacterial
-
Cytokines
-
Interleukin-12 Subunit p40
-
Protein Subunits
-
RNA, Messenger
-
Tumor Necrosis Factor-alpha
-
Interleukin-12
-
Interferon-gamma